Brief introduction of SILAC
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Stable isotope labeling using amino acids in cell culture (SILAC) is a powerful method based on mass spectrometry that identifies and quantifies relative differential changes in protein abundance. It was first used in quantitative proteomics in 2002 and provides accurate relative quantification without any chemical derivatization or manipulation.
Stable isotope labeling using amino acids in cell culture (SILAC) is a powerful method based on mass spectrometry that identifies and quantifies relative differential changes in protein abundance. It was first used in quantitative proteomics in 2002 and provides accurate relative quantification without any chemical derivatization or manipulation.
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