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This episode is part of a larger series of episodes examining the sensitivity and specificity of amplicon sequence variants (ASVs), also known as exact sequence variants (ESVs) and operational taxonomic units (OTUs). ASVs are becoming increasingly popular for analyzing microbial communities using 16S rRNA gene sequences. Proponents believe they should replace operational taxonomic units (OTUs). What do you think? Pat demonstrates these concepts by coding live on the command line interface using GitHub Flow, Make, and RStudio.
The accompanying blog post can be found at http://www.riffomonas.org/code_club/2021-03-01-submission
You can also find full tutorials for learning R with the neatverse using…
Microbial ecology data: http://www.riffomonas.org/minimalR/
General data: http://www.riffomonas.org/generalR/
0:00 Introduction
4:05 Transfer ownership and rename the repository
8:40 Application letter
10:13 Upload files and enter metadata
6:30 PM Post a preprint via the ASM system
21:05 Suggestions from editors and reviewers
25:22 Submission!
28:44 Conclusion
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