Join The Amoeba Sisters as they explain the biotechnology technique of PCR. This video goes over the basics of how PCR works, as well as two examples of its possible uses.

Table of contents:
00:00 Introduction
1:34 How does PCR work?
4:31 Why use PCR?
5:10 rRT-PCR testing for SARS-CoV-2 (virus that causes COVID-19)
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Factual references (and excellent for learning more details on this topic):

Clark, Mary Ann, et al. “17.1 Biotechnology – Biology 2e OpenStax.” Biology 2e, OpenStax, March 28, 2018, openstax.org/books/biology-2e/pages/17-1-biotechnology

https://www.fda.gov/consumers/consumer-updates/coronavirus-testing-basics

https://www.fda.gov/media/136151/download

*General length of a primer in base pairs from NIH https://www.genome.gov/genetics-glossary/Primer
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FURTHER READING SUGGESTIONS:

About RT-PCR for SARS-CoV-2:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7197457/
https://jamanetwork.com/journals/jama/fullarticle/2765837

About fluorescent probes in PCR:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC52277/?page1
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7148886/

What about the limitations or disadvantages of PCR?
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4102308/

How about more applications for PCR (as well as more information about the Nobel Prize):
https://www.nobelprize.org/prizes/chemistry/1993/press-release/
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