For more information, visit http://www.bio-rad.com/yt/6/PDQuest.
This tutorial explains how to optimize the detection of protein spots on 2D gels using Bio-Rad's PDQuest 2D analysis software. Topics covered include the selection of parameters to guide spot analysis, such as spot shape and intensity, display of certain proteins on individual gels, and spot selection criteria for further analysis. Processing options such as warping the gel and removing speckles are also discussed.

Learn how to independently adjust the detection sensitivity, magnitude scale, and minimum peak values to fine-tune the dynamic detection range with instant visual confirmation. The principles of background subtraction and stripe removal are demonstrated, and instructions are given for saving parameter sets for reuse with gels used under the same experimental conditions with similar amounts of protein.

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