In this video you will learn some important practical considerations and quick tips to keep in mind when preparing your PCR Mastermix.

This takes into account the same scenario as in the video on PCR calculations: an experiment to clone the same gene from multiple bacterial strains, where the Master Mix is prepared first and the strain-specific template DNA is later added separately to each PCR tube (this step is not shown).

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The life science business of Merck KGaA, Darmstadt, Germany, operates as MilliporeSigma in the US and Canada.

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