In this video I explain quality control in RNA-Seq analysis.

We can use any quality control tool, for example FastQC, Trim Glore. FastQC is a tool used for the quality assessment of NGS data. After checking the quality of the FastQC data, we will use the Fastp tool to trim the adapter sequences and filter the data. Fastp is a data preprocessing tool. It has features for quality control, adapter trimming, quality filtering, read pruning and multi-threading. After cropping the data, we check the data quality again using FastQC.

Quality control and data preprocessing are important for data analysis because raw data produced after sequencing must be processed so that the results do not produce false positive and false negative results. Data preprocessing not only evaluates each analysis step, but also reduces the number of low-quality sequence reads or adapter-contaminated sequence reads.

Learn BioCode's hands-on RNA sequencing with Linux & R course. This command line-based practical RNA-Seq data analysis with Linux & R is absolutely suitable for beginners who have no previous experience with Linux, R or even RNA-Seq.

In this course you will learn:
-Introduction to RNA-Seq, NGS
– In-depth knowledge of R & Linux
-Command line tools for RNA-Seq
-Quality control and trimming
– Mapping and evaluating alignment
-Discover DEGs with DESeq2, edgeR & Ballgown
-Functional analysis with gene ontology and KEGG pathways

If you would like to learn more about RNA sequencing, please DM us and we can help you get started. BioCode provides an interactive platform to learn biological programming in Python & R, bioinformatics techniques, tools, databases and biological data analysis in a cooperative manner, covering both theoretical and practical aspects of the computational biology topics. BioCode provides you with videos on every topic along with exercises. BioCode gives you the opportunity to learn at your pace and on your own schedule. With each video, BioCode provides you with the transcripts and PowerPoint presentation on that topic. If you have any questions during the lectures, there is a special section where you can ask your teacher questions.

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